发表于 2017-03-27 13:07
Abstract: The fundamental units of an organism are biological cells, which are involved in the development and maintenance of the hierarchical structures of the organism. Communication between neighboring cells, mediated by physical contact and diffusive signaling, regulate normal cellular functions. The functional characteristics of a single cell are determined by its genetic coding as well as its microenvironment. Due to the extreme complexity of in vivo environments, our understanding of the cellular functions and cell-cell interactions are heavily dependent on cell culture. Numerous studies have demonstrated the importance of cell arrangements for the functions of the cells in culture. To understand the biological mechanisms at cellular level in a cell culture, it is necessary to assess the temporospatial interactions of a single cell with its surroundings, including neighboring cells and adjacent extracellular matrix. However, there is no commercially available instrument that can be used to achieve the site- and time-specific placement of an individual cell in a cell culture. There is a critical need to develop a single cell manipulation system for the study of contact and diffusion-mediated communication among neighboring cells. In this talk, I will describe a novel laser cell micromanipulation technique based on the optical force generated by a weakly focused laser beam. I will demonstrate several applications of this technique including 1) the formation of a neuronal circuit on microelectrode arrays, 2) alignment of adult myocytes, 3) mitochondrial transfer through a nanotube formed between a single stem cell-cardiomyocyte cell pair, and myofibrillogenesis in a single cardiomyocyte laser patterned in a microgroove.
CV：Dr. Bruce Gao received his BS degree in Physical Electronics and Optoelectronics in 1985 and his MS degree in Applied Laser Physics in 1988 both from Tianjin University, China. He received his PhD degree in Biomedical Engineering at the University of Miami in 1999, followed a three-year post-doctorial training in cell and tissue engineering at the University of Minnesota. He is currently a professor in the Department of Bioengineering at Clemson University. His long-term research goal is to understand the mechanisms used by various cell types to form a functional tissue. To achieve this goal, he has been focusing his research on microfabrication, laser cell micromanipulation, coherent light and nonlinear optics-based 3D imaging and multiscale modeling to explore cell-cell interactions in an engineered microenvironment, such as a biochip. His current research projects include: 1) Single neuron-based cell biochip for the investigation of developmental neurotoxicity (NIH SC INBRE); 2) Electrical and mechanical coupling between cardiogenic bone-marrow stem cells and cardiac cells (NIH R01 and SC COBRE); and 3) Laser microbeam and microfluidics hybrid, high throughput and label-free cell sorting (NSF MRI).
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